| Method | Application field | MP code | Ref. | Accreditation |
| Competitive ELISA test, for detection of antibodies against NP of Influenza A virus | Pig sera | MP 04/039 | de Boer GF, Back W, Osterhaus AD. An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal species. Arch Virol. 1990;115(1-2):47-61. | No |
| Haemoagglutination inhibition test, for titration of antibodies against swine influenza virus (H1N1,H1N2,H3N2 and H1N1pdm) | Pig sera | MP 04/094 | OIE Manual of diagnostic tests and vaccines for terrestrial animals, ed 2015, cap 2.8.7 Kendall AP, Pereira MS, Skehel JJ. Concepts and procedures for laboratory-based influenza surveillance 1982. |
No |
| Isolation of swine influenza virus by SPF eggs inoculation | Swine clinical samples, organs, swabs, oral fluids | MP 05/030 | OIE Manual of diagnostic tests and vaccines for terrestrial animals, ed 2015, cap 2.8.7 | No |
| Isolation of swine influenza virus by cell culture inoculation | Swine clinical samples, organs, swabs, oral fluids | MP 05/033 | OIE Manual for Terrestrial Animals 2015 cap 2.8.7 par B.1.1.2+B.1.1.4 | No |
| Real-Time PCR for detection of Matrix (M)gene of influenza A virus | Swine clinical samples, organs, swabs, oral fluids | MP 09/032 | OIE Manual of diagnostic tests and vaccines for terrestrial animals, ed 2015, cap 2.8.7 SLOMKA M.J., DENSHAM A.L., COWARD V.J., ESSEN S., BROOKES S.M., IRVINE R.M., SPACKMAN E., RIDGEON J., GARDNER R., HANNA A., SUAREZ D.L. & BROWN I.H. (2010). Influenza Other Respir. Viruses, 4, 277-293. |
Yes |
| RT-PCR MULTIPLEX for swine influenza virus typing (H1N1,H1N2,H3N2 and H1N1pdm) | Swine clinical samples, organs, swabs, oral fluids, isolated virus | MP 09/117 | Chiapponi C, Moreno A, Barbieri I, Merenda M, Foni E. J Virol Methods. 2012 Sep;184(1-2):117-20. 4. LeBlanc JJ, Li Y, Bastien N, Forward KR, Davidson RJ, Hatchette TF. J Clin Microbiol. 2009 Dec;47(12):3805-13. |
No |
| Haemoagglutination inhibition test, for swine influenza virus antigenic typing | Isolated viruses | MP 05/038 | OIE Manual for Terrestrial Animals 2015 cap 2.8.7 par B.1.2.1 | no |
Diagnostic reagents can be provided with specific Material transfer agreement:
- IAV-S strains, genetically characterized
- Positive and negative reference sera
- RNA from virus strains
further technical information contact: Dr. Chiara Chiapponi
Tel +39 0521 293733- Fax +39 0521 29353
Email: chiara.chiapponi@izsler.it