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Istituto Zooprofilattico Sperimentale "Bruno Ubertini" - Activity
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 Home Page » Servizi » Centri di Referenza » Internazionali » OIE Reference Laboratory for Rabbit Haemorrhagic Disease » Activity 
Ultimo aggiornamento: Monday 08 June 2020
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  1. Specialized diagnostic activity with different diagnostic methods (see below)
  2. Diagnostic approach in the case of outbreaks and definition of control procedures and guidelines for the eradication of the diseases in infected farms
  3. Legislative support for the Ministry of Health and Veterinary Regional Officers
  4. Capability of experimental trials (i.e. for assessing vaccine potency and efficacy) in BL3 confinement animal facilities.  In the laboratories and animal facilities of IZSLER the rules and provisions given in the chapter 1.1.3."Biosafety and biosecurity: standard for managing biological risk in the veterinary laboratory and animal facilities" of the "OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals" are applied.
IZSLER uses a Management Quality Control System for its services, aimed at giving reliable analytical results, which are based on the international UNI CEI EN ISO/IEC 17025 for accreditation of testing and calibration laboratories.
The adoption of such standards guarantees the technical competence and the impartiality of the laboratory in performing the analyses. This, indeed, become an element for the reciprocal recognition of the validity of the results at international level.
Today, IZSLER is enrolled on the register of the Accredia (the national organization recognized for the accreditation of testing and calibration laboratories) as n. 0148 and includes 17 locations. The list of all accredited methods  of IZSLER could be found here.

Most of the methods for serological and virologic diagnosis of Myxomatosis have been developed "in house" and then standardized. They are listed in Table 1. 
 
Table 1: List of methods used to diagnose lagoviruses
 
Name of the method Scope IZSLER Code
RHD ELISA sandwich * Virologic diagnosis RHD MP 05/027
MP 05/027A
RHD PCR1 MP 09/089
RHD Western blot  
Haemoagglutination test  
EBHS ELISA sandwich Virologic diagnosis EBHS MP 05/028
EBHS PCR MP 09/097
EBHS Western blot  
Immuno-histochemical methods on thin section Virological diagnosis RHD/EBHS MP 07/003
Drop method - Negative staining Electron Microscopy  Diagnosis of viral agents MP 05/002
Ultracentrifugation method - Negative staining Electron Microscopy - MP 05/003
Immune-electron-microscopy (IEM) Negative staining Electron Microscopy - Diagnosis and typing of viral agents MP 05/023
Total antibodies - MAbs2 based competitive RHDV ELISA (kit IZS-BS) * Antibodies anti-RHDV MP 04/087
MP 04/087A
Total antibodies - MAbs2 based competitive RHDV2 ELISA (kit IZS-BS) Antibodies anti-RHDV2 MP 04/158
Total antibodies - MAbs2 based competitive EBHS ELISA (kit IZS-BS) Antibodies anti-EBHSV MP 04/093
IgA isotype - RHD ELISA * RHDV/RHDV2 IgA Antibodies MP 04/134
MP 04/134A
IgG isotype - RHD ELISA * RHDV/RHDV2 IgG Antibodies
IgM isotype - RHD ELISA * RHDV/RHDV2 IgM Antibodies
* Methods described in the OIE Manual for Terrestrial Animals cap. 2.6.2
Different PCR protocols are available including those able to distinguish RHDV classical strains from RHDV2 (RHDVFra2010)
Specific anti-RHDV, anti RHDV2 and anti-EBHSV MAbs were produced and characterized before their use in competitive ELISA