Activity

Ultimo aggiornamento: 20 Novembre 2019
  1. Specialized diagnostic activity with different diagnostic methods (see below)
  2. Diagnostic approach in the case of outbreaks and definition of control procedures and guidelines for the eradication of the diseases in infected farms
  3. Legislative support for the Ministry of Health and Veterinary Regional Officers
  4. Capability of experimental trials (i.e. for assessing vaccine potency and efficacy) in BL3 confinement animal facilities. In the laboratories and animal facilities of IZSLER the rules and provisions given in the chapter 1.1.3.”Biosafety and biosecurity: standard for managing biological risk in the veterinary laboratory and animal facilities” of the “OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals” are applied.

IZSLER uses a Management Quality Control System for its services, aimed at giving reliable analytical results, which are based on the international UNI CEI EN ISO/IEC 17025 for accreditation of testing and calibration laboratories.
The adoption of such standards guarantees the technical competence and the impartiality of the laboratory in performing the analyses. This, indeed, become an element for the reciprocal recognition of the validity of the results at international level.
Today, IZSLER is enrolled on the register of the Accredia (the national organization recognized for the accreditation of testing and calibration laboratories) as n. 0148 and includes 17 locations and more than 1600 accredited methods. The list of accredited methods could be found here.

Most of the methods for serological and virologic diagnosis of Myxomatosis have been developed “in house” and then standardized and listed among accredited methods (Table 1).

Table 1: List of methods used to diagnose myxomatosis

Name of the method Scope IZSLER Code Method type
Immunofluorescence Virologic diagnosis NK 758 Internal
Immunohistochemical methods Virologic diagnosis MP 07/003 Internal
Cell culture isolation Virologic diagnosis MP 05/032
MP 05/032 H
Internal
Immunoperoxidase Virologic diagnosis NK 2444 Internal
Drop method – Negative staining Electron Microscopy Diagnosis of viral agents MP 05/002 Internal
Ultracentrifugation method – Negative staining Electron Microscopy MP 05/003 Internal
Immune-electron-microscopy (IEM) -Negative staining Electron Microscopy Diagnosis and typing of viral agents MP 05/023 Internal
PCR1 Virologic diagnosis MP 09/146 Internal
Real Time PCR Virologic diagnosis MP 09/194 Internal
Total antibodies – MAbs2 based competitive ELISA – Ag protein m71L (kit IZS-BS) Antibodies anti-myxomatosis MP 04/052 Internal

1Different PCR protocols are available including those able to distinguish wild from vaccinal strains (SG33 and Borghi)
2Specific anti-myxomavirus MAbs were produced and preliminary characterized before their use in competitive ELISA